Unidos contra el Parkinson

Las células madre adultas se originan en una parte del cerebro diferente de la que comúnmente se había venido señalando, y con la estimulación adecuada pueden producir nuevas células cerebrales para reemplazar a las perdidas por enfermedades o lesiones cerebrales, según demuestra un estudio realizado por científicos de la Universidad de California en Irvine.

Las evidencias muestran de manera muy sólida que las verdaderas células madre en el cerebro de los mamíferos son las células ependimarias que forran los ventrículos en el cerebro y en la médula espinal, en vez de las células en la zona subventricular, como creían anteriormente los biólogos.
Los ventrículos cerebrales son cámaras huecas llenas de fluido que sostienen al tejido cerebral, y están cubiertos por una capa de estas células ependimarias.
Es crucial conocer la fuente de las células cuando se desarrollan terapias basadas en células madre. Además, el saber que estas células normalmente durmientes pueden ser inducidas a realizar la división celular abre el camino hacia terapias futuras en las que las propias células madre del paciente produzcan nuevas células cerebrales para tratar enfermedades y lesiones neurológicas, como por ejemplo el mal de Parkinson, los derrames cerebrales o las heridas neurológicas provocadas por accidentes y otras causas no naturales.

"Con tal terapia, sabríamos a qué células en el cuerpo activar, y su descendencia tendría todas las propiedades necesarias para reemplazar a las células dañadas o ausentes", explica Darius Gleason, autor principal del estudio. "Este es un enfoque muy prometedor de la terapia con células madre".
Las células madre son las "células primordiales" que producen todas las demás células especializadas del cuerpo humano. Si los científicos pudieran controlar la producción y diferenciación de las células madre, serían capaces de utilizarlas para reemplazar los tejidos dañados.
Uno de los objetivos de la investigación en células madre es mejorar el transplante de las mismas. El proceso usual pasa por inyectar en el cuerpo del paciente células sanas que pueden encajar genéticamente con las del paciente o no. El transplante de células madre con diferencias genéticas importantes requiere el empleo de fármacos para evitar que el cuerpo rechace el tratamiento.
Pero si se puede trabajar habitualmente con las células madre del propio paciente, se eliminaría la necesidad de transplantes y de fármacos inmunosupresores, lo que sería sin duda una mejor alternativa.

Articulo original:

Adult stem cells activated in mammalian brain
Locating cell origin provides foundation for brain injury cure
Irvine, Calif., July 24, 2008

Adult stem cells originate in a different part of the brain than is commonly believed, and with proper stimulation they can produce new brain cells to replace those lost to disease or injury, a study by UC Irvine scientists has shown.

Evidence strongly shows that the true stem cells in the mammalian brain are the ependymal cells that line the ventricles in the brain and spinal cord, rather than cells in the subventricular zone as biologists previously believed. Brain ventricles are hollow chambers filled with fluid that supports brain tissue, and a layer of ependymal cells lines these ventricles.

Knowing the cell source is crucial when developing stem cell-based therapies. Additionally, knowing that these normally dormant cells can be coaxed into dividing lays the groundwork for future therapies in which a patient's own stem cells produce new brain cells to treat neurological disorders and injuries such as Parkinson's disease, stroke or traumatic brain injury.

"With such a therapy, we would know which cells in the body to target for activation, and their offspring would have all the properties necessary to replace damaged or missing cells," said Darius Gleason, lead author of the study and a graduate student in the Department of Developmental and Cell Biology. "It is a very promising approach to stem cell therapy."

Study results appear this month online in the journal Neuroscience.

Stem cells are the "master cells" that produce each of the specialized cells within the human body. If researchers could control the production and differentiation of stem cells, they may be able to use them to replace damaged tissues.

One focus of stem cell research is transplantation, which entails injecting into the body healthy cells that may or may not genetically match the patient. Transplantation of nonmatching stem cells requires the use of drugs to prevent the body from rejecting the treatment.

But, working with a patient's own cells would eliminate the need for transplantation and immunosuppressant drugs and may be a better alternative, scientists say. Ependymal cells line the fluid-filled ventricles, so a drug to activate the cells could theoretically travel through this fluid directly to the stem cells.

"The cells already match your brain completely since they have the same genetic make-up. That is a huge advantage over any other approach that uses cells from a donor," Gleason said. "If they are your cells, then all we are doing is helping your body fix itself. We're not reinventing the repair process."

In this study, Gleason and Peter Bryant, developmental and cell biology professor, used rats treated to develop the animal equivalent of Parkinson's disease. They chose this type of rat because in a previous study by UCI collaborator James Fallon, a small protein given to the brain-damaged rats sparked a rapid and massive production and migration of new cells, and significantly improved motor behavior.

First, the UCI researchers sought to determine the true location of stem cells in the rats by looking for polarized cells, which have different sets of proteins on opposite sides so that when one divides it can produce two different products. Polarization gives rise to asymmetric cell division, which produces one copy of the parent and a second cell that is programmed to turn into another cell type. Asymmetric cell division is the defining characteristic of a stem cell.

On rat brain samples, the researchers applied antibodies to identify proteins that may be involved in asymmetric cell division, and they found that polarization exists on the ependymal cells. "It couldn't have been a stronger signal or clearer message. We could see that the only cells undergoing asymmetric cell division were the ependymal cells," Gleason said.

Next, they gave a drug to induce cell division in the rats and examined their brains at intervals ranging from one to 28 days after the treatment. At each interval, they counted cells that were dividing in the ependymal layer. They found the most division at 28 days, when about one-quarter of the ependymal cells were dividing. Previous studies by researchers at other institutions were successful in getting only a few cells to divide in that layer.

"One interpretation of previous studies is there are scattered stem cells in the ependymal layer, and it is hard to locate them," Bryant said. "But we believe that all of the ependymal cells are stem cells, and that they all have the ability to be activated."

Researchers don't know yet what sparks cell division at the molecular level, but learning that process and how to control it could lead to a safe, effective stem cell therapy.

Fallon, psychiatry and human behavior professor, and researchers Magda Guerra and Jian-Chang Liu contributed to this study. All of the scientists are affiliated with the UCI Sue and Bill Gross Stem Cell Research Center.

Gleason's work is supported by a stem cell training grant from the California Institute for Regenerative Medicine. The UCI Office of Research, the Optical Biology Core in the Developmental Biology Center, a gift from the Joseph's Foundation, and the UC MEXUS-CONACYT Postdoctoral Research Program also supported this study.

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